Enzymes and Inhibitors
Thermo Scientific™ Pfl23II (BsiWI) (3 U/μL)
Cut at C^GTACG sites with Pfl23II (BsiWI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BsiWI, PspLI).
Thermo Scientific™ Bsp119I (BstBI) (10 U/μL)
Cut at TT^CGAA sites with Bsp119I (BstBI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuIm).
R&D Systems™ Pan Caspase Inhibitor Z-VAD-FMK
Used as cell-permeable, irreversible inhibitor of caspase activity. R&D Systems Pan Caspase Inhibitor Z-VAD-FMK binds to the active site of activated proteases irreversibly and inhibits apoptosis.
Invitrogen™ T4 DNA Ligase (1 U/μL)
Ideal for cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Thermo Scientific™ PfoI (10 U/μL)
Cut at T^CCNGGA sites with PfoI restriction enzyme recognizes and cuts best at 37°C in Tango buffer.
Invitrogen™ Anza™ 8 XhoI
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
Thermo Scientific™ Bst1107I (BstZ17I) (10 U/μL)
Cut at GTA^TAC sites with Bst1107I (BstZ17I) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: BssNAI, BstZ17I).
Thermo Scientific™ FastDigest Esp3I (IIs class)
Cut at CGTCTC(1/5)^ sites with Thermo Scientific™ FastDigest™ BsmBI (Esp3I), which peforms best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Thermo Scientific™ FastDigest SaqAI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Supplied with Terminal Deoxynucleotidyl Transferase (TdT)
Thermo Scientific™ FastDigest NsbI
Cut at TGC^GCA sites with Thermo Scientific™ FastDigest™ FspI (NsbI), which performs best using universal FastDigest Buffer (Isoschizomers: Acc16I, AviII).
Thermo Scientific™ Klenow Fragment, exo– (5 U/μL)
Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.
Thermo Scientific™ FastDigest MreI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Thermo Scientific™ FastDigest FspAI
Cut at RTGC^GCAY sites with Thermo Scientific™ FastDigest™ FspAI, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.